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In QuickBooks, click on Banking->Use Register -- and choose the bank register you are working with.
Once the bank register appears, click on 'Print'->enter the date range->OK
You'll choose to Print to 'File' and save as a 'Comma delimited file'.
Save the file to a location and with a name that you can recognize.
Once the file is created, you'll import in ACH Universal (assuming Profiles have been set up) or Bank Positive Pay.