Pre-notes are transactions containing zero-dollar amounts.
A pre-note is considered a test transaction that is sent before the actual transaction is transmitted to the bank.

This help page covers Excel/csv file created pre-notes -as well as profile (manual) created pre-notes.
QuickBooks Online uses profiles (and this page).

For QuickBooks, see QuickBooks Pre-Note

Creating a Pre-notification

When importing transactions as a file, ACH Universal will treat all zero-dollar amounts as pre-notes.  It will create a debit or credit pre-note based on your default selection during the Account Setup. 

File Level Import

In creating a pre-note, you can import or enter all transactions as you normally would.  

Manually Entered Pre-notification

Similar to transactions, a pre-note can also be entered manually.

From the Home screen select 'Add A Record'. 

Fill out all fields exactly as you would for a dollar transaction, except the amount must be $0.00.  Click OK. A dialog box will be displayed to confirm that you intended to create a pre-note.  (This is to verify that the amount was intended to be zero and was not left blank unintentionally).  Click 'Yes' to create the pre-note.

You can import or enter all other types of transactions as you normally would.  

During transmission, on the 'Transmit to Bank' screen you will see the number of pre-note transactions listed in the summary information in the 'Pre-Note' column.