It is necessary for transactions to appear in the QuickBooks Bank Register for them to import to ACH Universal. In other words, bills must be paid, checks written, and deposits processed in QuickBooks first.
You'll go to the Quickbooks Tab, and select the transactions to process by date range, and click "View Transactions".
ACH Universal will automatically populate the employees, customers, and/or vendors for processing within that date range. If there are no stored profiles for the individual/entity in ACH Universal, the checkbox "Missing banking profile info" will be marked.
See "QuickBooks Online - Profiles" for additional information.